Here are some common problems that can occur with fluorescence microscopes and how to troubleshoot them:

• No image: Check to make sure that the light source is turned on and that the filters are properly aligned. Also, make sure that the sample is properly mounted and that the objective lens is in focus.
• Fluorescence is weak: Check the concentration of the fluorescent dye and the thickness of the sample. You may also need to increase the exposure time.
• Fluorescence is uneven: This can be caused by uneven illumination or by uneven distribution of the fluorescent dye in the sample. Try adjusting the light source or the sample.
• Photobleaching: This is the process by which fluorescent molecules lose their ability to fluoresce after exposure to light. To reduce photobleaching, use a lower light intensity and a shorter exposure time.
• Background noise: This is the presence of unwanted light in the image. To reduce background noise, use a higher quality filter set and a lower light intensity.

Start with the fundamentals. Verify that the microscope is correctly put together and that each of its parts is functioning as it should. Be methodical. When troubleshooting, make an effort to narrow the issue to a particular element or procedure. Chongqing Scope Instrument Co., Ltd. has professional machines, technology and service. If you want more information, please click here.