Knowing how to properly load a pipette tip is important for ensuring accurate and precise results and preventing contamination. To properly load a pipette tip, follow these steps:
- Choose the correct pipette tip. Verify that the pipette tip you select is appropriate for the pipette type and liquid volume you are transferring.
- Grip the pipette tip by the flange. The pipette tip's flange is its wider portion. To guarantee a good seal and avoid contamination, hold the pipette tip by the flange.
- Insert the pipette tip into the pipette shaft. Verify that the pipette shaft and tip are completely inserted. The pipette shaft and tip should not be separated in any way.
- Gently press down on the plunger to create a vacuum. You can guarantee that the pipette tip and liquid sample will be tightly sealed by doing this.
- Release the plunger to aspirate the liquid sample. Take care not to aspirate the liquid sample too much. The pipette tip's liquid level ought to be just below the graduation mark.
- Dispense the liquid sample by slowly pressing down on the plunger. Take care not to administer the liquid sample too rapidly as this may cause unreliable findings.
- Eject the pipette tip by pressing the ejector button. The pipette shaft should allow the tip to be neatly ejected.
You can make sure that your pipette tips are loaded correctly and that your results are precise and accurate by following these steps.
