How Should Cell Culture Consumables Be Sterilized?

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How Should Cell Culture Consumables Be Sterilized?

Cell culture consumables must be sterile to prevent contamination of cell cultures. Contamination can lead to inaccurate experimental results and loss of valuable cell lines. Here are common sterilization methods:

  • Autoclaving:This is a high-pressure steam sterilization method suitable for most materials, including glass and polystyrene. It is the most common method for sterilizing cell culture consumables.
  • Gamma Irradiation:This method uses ionizing radiation to sterilize materials. It is suitable for heat-sensitive materials and can be used to sterilize large quantities of consumables.
  • Ethylene Oxide Gas Sterilization:This method is suitable for heat-sensitive materials, such as plasticware and certain types of filters. However, it requires special equipment and can be time-consuming.

The choice of sterilization method depends on the type of consumable and the desired level of sterility. Some consumables, such as plasticware, may be pre-sterilized by the manufacturer.

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